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Trakia Journsl of Science, 2016, vol.14, n.1 >

Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/113

Title: Callus induction and plant regeneration in lemon verbena ( Lippia Citrodora L. ), an important medicinal plant
Authors: Boustani, A.
Omidi, M.
Torabi, S.
Zarekarizi, A.
Keywords: Lippia citriodora L.
plant regeneration
medicinal plant
Callus induction
root induction
N6-benzyladenine
Issue Date: 2016
Citation: A. Boustani, M. Omidi, S. Torabi, A. Zarekarizi. Callus induction and plant regeneration in lemon verbena ( Lippia Citrodora L. ), an important medicinal plant, 14, 2016, N 1, 30-38
Abstract: In order to investigate the optimization of Lippia citriodora L., a richly scented medicinal plant, in different aspects of tissue culture; young stem cuttings were cultured on Murashige and Skoog (MS) medium containing 0.01 mg l-1 indole-3-butyric acid (IBA) and 0.5 mg l-1 N6-benzyladenine (BA) to produces sterilized seedling. After 21 days, three types of explants including apical meristem, young stems carrying buds and young petioles with part of leaf were excised from the growing seedling and cultured on MS medium containing various combinations of α-naphthalene acetic acid (NAA), 2, 4-dichlorophenoxyacetic acid (2,4-D) and BA for callus induction. Callus formation was occurred in several combinations of growth regulators among them 5 treatments have found as the most effective mediums for callus formation percentage. The highest callus wet weight was observed in young petiole explants with MS medium supplemented with 0.5 mg l-1 2,4-D and 1 mg l-1 BA, and the best callus dry weight was obtained in meristem explants which were placed on MS medium, supplemented with 0.5 mg l-1 NAA and 2 mg l-1 BA. Visible calli were transferred to MS and quarter MS medium supplemented with different concentrations of 2,4-D, BA, NAA and GA3 to shoot regeneration. Only calli derived from young stems were regenerated. Furthermore, MS medium with 2 mg l-1 BA alone was the most effective treatment for regeneration percentage. Many of regenerated calli were rooted in their shoot organogenesis medium and the rest of them were sub-cultured in MS medium supplemented with activated carbon thus root emergence was induced in all calli samples. The rooted shoots were acclimatized and transferred into soil successfully.
URI: http://hdl.handle.net/123456789/113
ISSN: 1313-3551
Appears in Collections:Trakia Journsl of Science, 2016, vol.14, n.1

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